The Zoo has two game breeding centres in Lichtenburg, in the North West Province and Mokopane, in the Limpopo Province.

Lichtenburg Biodiversity Conservation Centre
The Centre breeds many endangered species including white rhino, Pere David's deer, Cape mountain zebra, scimitar-horned oryx and Arabian oryx.

Mokopane Biodiversity Conservation Centre
The Centre is home to a wide variety of wildlife species originating from Africa, South America and South East Asia.